Bradford Assay | Protein, Protocol & Methods
5:39
Bradford Assay | Protein, Protocol & Methods
111K viewsMay 22, 2018
Study.comLaura Foist
SOLVED: A Bradford assay was performed to determine the protein content in some samples. A calibration curve below was prepared from the following standard solutions (table below). A least squares line was fitted to the calibration data; the equation of the line is shown on the graph:(s)Asgs VS ug Protein6S6SV14protein ug absorbance120.466 0.676 0.883 1.086 1.280.8 2 0.6Y = 0.0218x 0.4706 R² = 0.99989.36 18.72 80.82 37.440.40.2302010ug ProteinFrom the fit, the following least squares paramete
SOLVED: A Bradford assay was performed to determine the protei…
Dec 13, 2023
numerade.com
Bradford method of protein quantification
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SOLVED: Pre-Lab QuestionsDefine a protein and describe one important function of proteins in the human body. The following data were obtained from a Bradford assay to determine the protein content in a sample:Protein content (μg)Absorbance value: 0.000, 0.022, 0.065, 0.106, 0.178, 0.299, 0.380, 0.472, 0.15010, 20, 30, 40, 50 (unknown)Use Excel, another program, or graph paper to produce a calibration curve based on the data. Use linear regression (or best-fit line) to obtain the slope of the li
SOLVED: Pre-Lab QuestionsDefine a protein and describe one import…
Apr 28, 2022
numerade.com
in the bradford protein determination the colour of dye changes from brown to blue when it binds to protein absorbance of light is measured at 595 nm protein pg 000 936 1872 2808 3744 absorb 73228
in the bradford protein determination the colour of dye ch…
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numerade.com
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