pMHC tetramers are multimeric peptide-MHC complexes that serve as reagents for analyzing antigen-specific T cells. The multimeric nature of tetramers increases the affinity between pMHC and TCR …

In the presence of streptavidin which has four biotin binding sites per molecule, four MHC monomers are joined together to form a tetramer. Whilst single MHC:peptide complexes and TCR bind weakly, …

The easYmer kit features a highly active formulation of MHC class I (MHC-I) molecules, which can be used to generate specific peptide-MHC class I monomers of your choice in your own laboratory.

Among published reports and feedback from our clients, class II tetramer staining reactions have been carried out at a range of temperatures (4°C to 37°C), concentrations (1-20 ug/ml) and times (20 min …

Specific cell staining is accomplished by incubating the sample with the T-Select MHC Tetramer reagent, then washing away excess Tetramer. The number of Tetramer positive lymphocytes is then …

Here, we describe an optimized protocol for chaperone-mediated exchange of a high-affinity placeholder peptide for an array of target peptides. Using this protocol, a pMHC-I tetramer library encompassing …

An MHC monomer is a sub-component of an MHC tetramer, made up of the MHC complex (α-chain plus β-2 microglobulin for class I or α-chain plus β-chain for class II) folded together with a specific peptide.