Nature

An efficient full-length cDNA amplification strategy based on bioinformatics technology and multiplexed PCR methods

The transcriptomes of increasing number of species have been sequenced by next generation sequencing (NGS) technique. However, the transcriptomes are always incomplete in length, especially the ends ...
Nature

Correcting PCR amplification errors in unique molecular identifiers to generate accurate numbers of sequencing molecules

Unique molecular identifiers are random oligonucleotide sequences that remove PCR amplification biases. However, the impact that PCR associated sequencing errors have on the accuracy of generating ...
The Scientist

Smart Race To The Finish: Clontech Enters Smart Race For cDNA Amplification

CLONTECH's SMART RACE cDNA amplification process diagram On your mark, get set, go! And the RACE is on to obtain that potentially elusive full-length cDNA for characterizing the best gene ever. In the ...
BioTechniques

RNA sequencing library preparation and construction

What is an RNA sequencing library? In all RNA-seq experiments, a library of cDNA fragments attached to adapters must be synthesized in order for sequencing to be successful. How do you prepare an RNA ...